扩展莫尼茨绦虫wnt基因在虫体不同发育体节的差异表达

doi:10.11843/j.issn.0366-6964.2016.12.019

Abstract

Abstract:

The mRNA relative expression and tissue distribution of wnt gene family wnt1, wnt2, wnt4, wnt5 and wnt11B in different proglottides of Moniezia expansa were investigated in the present study to provide basic information for exploring the roles of the wnt signaling pathway in development of M. expansa. The relative mRNA expression of wnt1, wnt2, wnt4, wnt5 and wnt11B in different proglottides of M. expansa were determined by qRT-PCR. Tissue location of wnt1, wnt2, wnt4, wnt5 and wnt11B in different proglottides of M. expansa were detected using in situ hybridization. The quantitative results showed that the gene family of wnt were transcribed in all proglottides of adult M. expansa. The transcription of wnt1, wnt4, wnt5 and wnt11B were higher in scolex than other proglottides, and in the gravid proglottides, the transcription of these genes was lowest. On the contrary, the transcription of wnt2 was lowest in the scolex, but higher in other proglottides. The in situ hybridization results showed that the wnt gene family mRNA were mainly distributed in suckers of scolex; in the mature proglottides, they were mainly distributed in ovary, testis and interproglottidal; in the immature and gravid proglottides, they were mainly distributed in interproglottidal. Based on these findings, the mRNA relative expression of these 5 wnt genes in scolex and immature proglottides was higher than in other proglottides, and mainly distributed in suckers, interproglottidal and germ cells. The results suggested that the Wnt signaling pathway may be involved in development of segments and germ cell in M. expansa, which remains to be further verified.

CLC Number:

S852.734

WANG Zheng-rong, ZHANG Yan-yan, BO Xin-wen, XU Xue-ping, XU Chun-sheng. Differential Expression of wnt Genes in Different Proglottides of Moniezia expansa[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(12): 2483-2492.

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Differential Expression of wnt Genes in Different Proglottides of Moniezia expansa

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